



All characters have specific statistics, but for Eden they are generated based on a number of variables and items for each run. The existing cast of playable characters is expanded with three new ones that can be unlocked: Azazel, Lazarus and Eden. The game retains its pixel based look, but adds a filter option to introducing smoothing. Next to the main runs there are 20 new challenge runs. Players can also choose between two difficulty levels and continue a run at a later time. These can now be copied to replay afterwards and there are 100 seeds with a special effect. The random environments are generated based on seeds. There is support for three save slots and there are new sound effects and music. Next to the existing three types of hearts that provide health, black hearts have been added. In addition to the single screen rooms of the original game, there are now some larger rooms with more enemies and where the screen scrolls. There are new environments and rooms, including new secrets rooms, new types of rocks and objects, mushrooms that hold pills, blue fires, new doors with additional requirements, a new final chapter, and the ability to upgrade shops by donating coins. There are also many new types of enemies and bosses. These provide new types of bullets, abilities, trinkets and familiars. The game contains more than 450 items of which 160 are new.

For the PS4 and PS Vita this is the first release of the game. It was initially started as a project to rewrite the game from scratch, to deal with the constraints of the Flash technology that affected performance, but it was eventually expanded into a semi-sequel with additional content and co-developed by Nicalis. The Binding of Isaac: Rebirth is both a remake of and a sequel to the roguelike action game The Binding of Isaac, also incorporating the expansion The Binding of Isaac: Wrath of the Lamb.
